BGI has a world-class multiomics technology platform, providing high-quality, industry-leading gene sequencing,
Protein spectroscopy, synthetic biology, biological database, cloud computing and other standardized technical services and comprehensive full-process solutions.
Human genetics and population chemistry research based on whole-genome resequencing can quickly screen out genetic variations within the genome, enabling genotypic diversity analysis, genetic evolution analysis, and screening for pathogenic and susceptible genes. With the development and popularization of high-throughput sequencing technology, whole-genome resequencing has become one of the most rapid and effective methods in the fields of human genetics, translational medicine, and population evolution. It can more fully mine genetic sequence differences and structural variations, including Single-base mutations, insertion and deletion mutations, copy number mutations, and structural mutations. Scanning and detecting mutation sites related to phenotypic differences, diseases, and evolution at the genome-wide level have great scientific value.
Whole-exome sequencing (WES) is the most frequently used genome sequencing method. Exons are protein coding regions of the human genome, and their DNA can be captured and enriched using sequence capture technology. Although the exon region is less than 2% of the whole genome, it focuses on the pathogenic mutation sites of most diseases. Compared to whole genome sequencing, whole exon sequencing is more economical and efficient. Exome sequencing is mainly used to identify and study structural variations in coding regions and UTR regions related to disease and population evolution. The combination of exon data provided by a large number of public databases is conducive to better explain the associations and pathogenic mechanisms between the resulting variant structures. BGI is the world's leading exon sequencing service provider, serving more than 130,000 exomes. Since the publication of the first domestic exon sequencing article in 2010, a total of 205 articles have been included, including 49 tumors, 94 single / rare disease types, and 43 complex diseases. A total of 95 articles were authored, a total of 53 articles were authors and corresponding authors, and a total of 45 articles with a score greater than 10 points.
Target region sequencing refers to a genomic analysis method that uses special probes to capture the DNA of a protein-coding region of interest to a customer or a specific sequence of a sequence and enrich it for high-throughput sequencing. This method can obtain the genetic information of the specified target area, which greatly improves the research efficiency of the specific target area in the genome and significantly reduces the research cost. By sequencing the target region, the candidate site or candidate gene can be verified, and the susceptible site within the candidate region or candidate gene can be further found, which is suitable for research such as candidate gene association analysis.
Single cell sequencing can detect the genetic and mutation information of a single cell from the DNA, RNA, and apparent levels. a) Single cell DNA sequencing b) Single cell RNA sequencing c) Single cell whole genome methylation
T / B cells are two major cell groups of the adaptive immune system. The cell surface receptor TCR / BCR has a region called the Complementary Determining Region (CDR), which contains CDR1, CDR2, and CDR3. Among them, CDR3 has the highest change. Identification plays a key role. BGI's immunology library sequencing is based on multiplex PCR and high-throughput sequencing technology, which analyzes the DNA / RNA sequence encoding the CDR3 region, obtains the body's immune characteristics, comprehensively evaluates the diversity of the immune system, and deeply digs into the immune database and diseases. relationship.
Proteomics is an important discipline in the post-genomic era. Its research objects are all proteins in the organism that are translated and perform life functions, the relationships between proteins, and the relationship between proteins and DNA, RNA and metabolites. Relationship. High-throughput and in-depth proteomics research based on liquid chromatography and tandem mass spectrometry (LC-MS / MS) can obtain protein expression profiles, protein post-translational modifications, quantitative differential protein expression profiles, peptide profiles, and quantitative differential peptide profiles , As well as protein interaction information, can be used in gene expression research, auxiliary gene annotation and correction, discovery of protein markers and peptide markers, mining of functional proteins and peptides, signal pathways and molecular mechanisms. Relying on the accumulation of many achievements in the field of genomics and transcriptomics, top instrument platforms, and high-end professionals, Huada has sufficient advantages in proteomics research and has achieved remarkable achievements (as of February 2019, Huada The mass spectrometry platform has published 85 research papers in the field of proteomics and metabolomics, with a cumulative impact factor of 432).
Metabolomics (Metababonomics / Metabolomics) is a newly developed discipline after genomics and proteomics. Its main goal is to quantitatively study the levels of metabolites in the body caused by external stimuli, physiological and pathological changes, and gene mutations. Multivariate dynamic response. The research object is small molecular substances with a relative molecular mass of less than 1000 Da, such as hydroxy acids, amino acids, sugars, lipids, ketones, organic acids, fatty acids, glyceroglycolipids, glycerophospholipids, etc. Metabolomics research is based on chromatography-mass spectrometry, collecting sample metabolite information, comparing the content of metabolites in different groups of samples from qualitative and quantitative levels, finding differential metabolites, and exploring metabolic pathways between differential metabolites It is widely used in biomarker discovery, traditional Chinese medicine research, disease diagnosis, and molecular mechanism discovery. Relying on the accumulation of many achievements in the field of genomics and transcriptomics, top instrument platforms, and high-end professionals, Huada has sufficient advantages in metabolomics research and has also achieved remarkable achievements. We are committed to helping our partners achieve better penetration, integration and complementarity throughout the life sciences and systems biology research.